Restriction Digest of Plasmid DNA
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a. Given the following restriction map of a cloned 10 kb piece of DNA, what size fragments would you see after digesting this linear DNA fragment with each of the enzymes or combinations of enzymes listed? (1) EcoRI, (2) BamHI, (3) EcoRI + HindIII, (4) BamHI + Pstl, and (5) EcoRI + BamHI. b. What fragments in the last three double digests would hybridize on a Southern blot with a probe made from the 4 kb Bam HI fragment? | Numerade
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